Editing sterol side chain reductase 2 gene (StSSR2) via CRISPR/Cas9 reduces the total steroidal glycoalkaloids in potato
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چکیده
منابع مشابه
Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato.
Potatoes (Solanum tuberosum) contain α-solanine and α-chaconine, two well-known toxic steroidal glycoalkaloids (SGAs). Sprouts and green tubers accumulate especially high levels of SGAs. Although SGAs were proposed to be biosynthesized from cholesterol, the biosynthetic pathway for plant cholesterol is poorly understood. Here, we identify sterol side chain reductase 2 (SSR2) from potato as a ke...
متن کاملReduction of Total Steroidal Glycoalkaloids in Potato Tubers Using Antisense Constructs of a Gene Encoding a Solanidine Glucosyl Transferase
Accumulation of steroidal glycoalkaloid (SGA) toxicants in potatoes affects food quality and safety. High levels of SGAs hamper breeding efforts to develop new varieties of potatoes with improved agronomic and post harvest properties. To speed breeding efforts for reducing SGAs in breeding lines and correcting flawed selections we are taking a molecular genetic approach to reduce SGAs. A cDNA e...
متن کاملSteroidal glycoalkaloids in Solanum chacoense.
Potato (Solanum tuberosum L.), a domesticated species that is the fourth most important world agricultural commodity, requires significant management to minimize the effects of herbivore and pathogen damage on crop yield. A wild relative, Solanum chacoense Bitt., has been of interest to plant breeders because it produces an abundance of novel steroidal glycoalkaloid compounds, leptines and lept...
متن کاملConversion of Exogenous Cholesterol into Glycoalkaloids in Potato Shoots, Using Two Methods for Sterol Solubilisation
Steroidal glycoalkaloids (SGA) are toxic secondary metabolites naturally occurring in the potato, as well as in certain other Solanaceous plant species, such as tomato, eggplant and pepper. To investigate the steroidal origin of SGA biosynthesis, cut potato shoots were fed cholesterol labelled with deuterium (D) in the sterol ring structure (D5- or D6-labelled), or side chain (D7-labelled), and...
متن کاملPotato Glycoalkaloids, Past Present and Future
The steroidal glycoalkaloids are naturally occurring specialty metabolites of questionable desirability in the vegetable crop, potato. Although glycoalkaloids undoubtedly originated under selection as feeding deterrents against herbivorous pests, they no longer function as the primary feeding deterrent. Moreover, due to their potential toxicity, guidelines persist as to the maximal allowable co...
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ژورنال
عنوان ژورنال: All Life
سال: 2021
ISSN: 2689-5293,2689-5307
DOI: 10.1080/26895293.2021.1925358